Human THR alpha (Thyroid Hormone Receptor Alpha) CLIA Kit
The Human THR alpha (Thyroid Hormone Receptor Alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of THR alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.Thyroid Hormone Receptor Alpha (THR alpha) is a key protein involved in regulating thyroid hormone signaling, playing a crucial role in metabolic processes, growth, and development.
Dysregulation of THR alpha has been implicated in various diseases including thyroid disorders, metabolic disorders, and certain cancers, making it a valuable biomarker for studying these conditions and potentially developing new treatments.Overall, the Human THR alpha CLIA Kit is an essential tool for researchers looking to accurately measure and study the role of THR alpha in various physiological and pathological processes.
Product Name:
Human THR alpha (Thyroid Hormone Receptor Alpha) CLIA Kit
SKU:
HUES01258
Target:
Human THR alpha (Thyroid Hormone Receptor Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human THRα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THRα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human THRα. You can calculate the concentration of Human THRα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
86-98
88-101
Average (%)
105
92
95
1:4
Range (%)
93-110
98-114
97-111
Average (%)
100
105
104
1:8
Range (%)
96-110
99-114
94-106
Average (%)
102
106
101
1:16
Range (%)
85-100
88-103
101-117
Average (%)
91
95
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
95-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.34
91.68
364.58
51.82
83.21
342.81
Standard deviation
4.38
8.67
28.66
6.71
7.26
36.99
C V (%)
9.25
9.46
7.86
12.95
8.72
10.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human THRα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human THRα in samples. No significant cross-reactivity or interference between Human THRα and analogues was observed.
