Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
The Human TIMP-2 (Tissue Inhibitors of Metalloproteinase-2) CLIA Kit is a reliable tool for the precise quantification of TIMP-2 levels in human tissues. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results across a variety of sample types, including serum, plasma, and cell culture supernatants.TIMP-2 is a critical regulator of extracellular matrix remodeling and cell migration, playing a key role in tissue homeostasis and pathological processes such as cancer and fibrosis.
By measuring TIMP-2 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and explore potential therapeutic interventions.The Human TIMP-2 CLIA Kit from AssayGenie is an essential resource for researchers studying metalloproteinase activity, tissue remodeling, and related pathologies. Its user-friendly design and robust performance make it a valuable asset for a wide range of research applications in the fields of cancer biology, cardiovascular research, and beyond.
Product Name:
Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
SKU:
HUES00830
Target:
Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TIMP-2. You can calculate the concentration of Human TIMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
84-97
101-120
Average (%)
105
91
110
1:4
Range (%)
91-105
90-104
94-108
Average (%)
98
97
100
1:8
Range (%)
86-96
99-115
100-116
Average (%)
91
105
108
1:16
Range (%)
97-113
97-113
83-97
Average (%)
103
105
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
106
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.39
196.54
787.38
103.57
214.28
763.36
Standard deviation
9.42
18.08
65.12
8.69
18.32
84.89
C V (%)
8.77
9.2
8.27
8.39
8.55
11.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-2 in samples. No significant cross-reactivity or interference between Human TIMP-2 and analogues was observed.
