Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
The Human TIMP-2 (Tissue Inhibitors of Metalloproteinase-2) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of TIMP-2 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.TIMP-2 is a key player in regulating the activity of metalloproteinases, enzymes that break down extracellular matrix proteins. Dysregulation of TIMP-2 has been implicated in various diseases, including cancer, cardiovascular conditions, and inflammatory disorders.
Understanding the levels of TIMP-2 in biological samples can provide valuable insights into disease mechanisms and potential therapeutic targets.Whether investigating the role of TIMP-2 in disease progression or monitoring its expression in response to treatments, the Human TIMP-2 ELISA Kit offers researchers a valuable tool for advancing their research. Trust in the accuracy and precision of this kit to uncover new insights into the complex biology of TIMP-2 and its implications for human health.
Product Name:
Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
SKU:
HUES02488
Target:
Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-2. You can calculate the concentration of Human TIMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
91-107
95-111
Average (%)
93
98
103
1:4
Range (%)
93-106
81-92
94-105
Average (%)
100
86
99
1:8
Range (%)
96-111
85-98
91-106
Average (%)
103
90
99
1:16
Range (%)
94-109
80-94
96-109
Average (%)
101
86
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
100
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.9
0.5
1.4
5.1
Standard deviation
0.03
0.07
0.24
0.03
0.08
0.17
C V (%)
6
5.38
4.9
6.0
5.71
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-2 in samples. No significant cross-reactivity or interference between Human TIMP-2 and analogues was observed.
