The Human TK1 (Thymidine Kinase 1) Soluble ELISA Kit is specifically designed for the precise measurement of TK1 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides accurate and consistent results, making it an invaluable tool for various research applications.TK1 is an important enzyme that plays a key role in DNA synthesis and cell proliferation. Abnormal levels of TK1 have been linked to various diseases, including cancer and other proliferative disorders.
As a result, TK1 serves as a critical biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human TK1 Soluble ELISA Kit offers researchers a reliable and efficient method for quantifying TK1 levels, enabling deeper insights into its biological functions and its implications in disease pathology.
Product Name:
Human TK1 (Thymidine Kinase 1, Soluble) ELISA Kit
SKU:
HUES03310
Target:
Human TK1 (Thymidine Kinase 1, Soluble)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TK1. You can calculate the concentration of Human TK1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
98-112
95-111
Average (%)
96
104
102
1:4
Range (%)
89-99
86-100
88-102
Average (%)
94
93
95
1:8
Range (%)
88-102
83-94
85-97
Average (%)
93
89
91
1:16
Range (%)
92-104
81-95
86-98
Average (%)
97
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.44
409.49
1921.23
190.86
404.41
2086.28
Standard deviation
11.96
22.89
102.4
13.32
18.44
76.57
C V (%)
6.15
5.59
5.33
6.98
4.56
3.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TK1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TK1 in samples. No significant cross-reactivity or interference between Human TK1 and analogues was observed.
