The Human TLN (Talin) CLIA Kit is a reliable and accurate tool for measuring talin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications. Talin is a key protein involved in cell adhesion and plays a crucial role in cell migration, proliferation, and signaling pathways. Dysregulation of talin has been linked to various diseases including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a biomarker for disease progression and treatment development.
By using the Human TLN (Talin) CLIA Kit, researchers can effectively study talin levels in different biological samples and gain valuable insights into its role in various physiological and pathological processes. This kit is a valuable tool for advancing research in cell biology, cancer biology, and other related fields.
Product Name:
Human TLN (Talin) CLIA Kit
SKU:
HUES00805
Target:
Human TLN (Talin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TLN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TLN. You can calculate the concentration of Human TLN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
85-101
101-112
Average (%)
91
92
106
1:4
Range (%)
99-112
96-109
91-106
Average (%)
106
103
97
1:8
Range (%)
91-106
92-105
95-113
Average (%)
97
98
103
1:16
Range (%)
99-112
93-107
97-110
Average (%)
104
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.74
319.84
831.6
94.72
305.9
888.85
Standard deviation
11.53
28.66
60.62
11.63
24.69
98.48
C V (%)
12.3
8.96
7.29
12.28
8.07
11.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TLN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLN in samples. No significant cross-reactivity or interference between Human TLN and analogues was observed.
