The Human TLR4 (Toll-Like Receptor 4) CLIA Kit is specifically designed for the accurate and sensitive detection of TLR4 levels in human serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, delivering consistent results for a variety of research applications.TLR4 is a key receptor in the innate immune system, playing a crucial role in the recognition of microbial pathogens and activation of inflammatory responses. Dysregulation of TLR4 signaling has been implicated in various diseases, including infectious diseases, autoimmune disorders, and chronic inflammatory conditions.
Therefore, monitoring TLR4 levels can provide valuable insights into disease progression and potential therapeutic targets.With its advanced technology and optimized protocols, the Human TLR4 CLIA Kit from AssayGenie is a valuable tool for researchers studying immune system function, inflammation, and infectious diseases. Trust in the accuracy and reliability of this kit to enhance your research discoveries and advancements in the field of immunology.
Product Name:
Human TLR4 (Toll-Like Receptor 4) CLIA Kit
SKU:
HUES00872
Target:
Human TLR4 (Toll-Like Receptor 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TLR4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TLR4. You can calculate the concentration of Human TLR4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
93-107
93-109
Average (%)
105
99
99
1:4
Range (%)
91-101
96-109
88-102
Average (%)
96
104
94
1:8
Range (%)
88-102
98-115
98-112
Average (%)
94
105
106
1:16
Range (%)
92-108
87-99
87-102
Average (%)
99
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.73
335.07
1584.16
207.3
367.47
1733.16
Standard deviation
20.79
30.79
127.52
21.25
40.16
191.86
C V (%)
10.73
9.19
8.05
10.25
10.93
11.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TLR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR4 in samples. No significant cross-reactivity or interference between Human TLR4 and analogues was observed.
