The Human TLR9 (Toll-Like Receptor 9) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TLR9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers in various fields.TLR9 is a critical component of the innate immune response, recognizing and responding to viral and bacterial DNA.
Dysregulation of TLR9 has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.With its high sensitivity and specificity, the Human TLR9 CLIA Kit from AssayGenie is the ideal choice for researchers looking to study the role of TLR9 in immune responses, infection, and inflammatory diseases.
Product Name:
Human TLR9 (Toll-Like Receptor 9) CLIA Kit
SKU:
HUES00567
Target:
Human TLR9 (Toll-Like Receptor 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TLR9/CD289. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR9/CD289 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR9/CD289, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TLR9/CD289. You can calculate the concentration of Human TLR9/CD289 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
87-103
95-110
Average (%)
93
94
102
1:4
Range (%)
87-99
88-103
87-104
Average (%)
93
95
95
1:8
Range (%)
96-109
91-105
89-102
Average (%)
101
96
96
1:16
Range (%)
96-109
93-106
103-118
Average (%)
103
100
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
99-117
107
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.9
185.85
900.19
105.07
201.53
909.75
Standard deviation
10.96
18.62
96.14
11.38
14.19
92.43
C V (%)
11.2
10.02
10.68
10.83
7.04
10.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TLR9/CD289 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR9/CD289 in samples. No significant cross-reactivity or interference between Human TLR9/CD289 and analogues was observed.
