The Human TLR9 (Toll-like Receptor 9) ELISA Kit is specifically designed for the accurate quantification of TLR9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.TLR9 is an important protein involved in the innate immune response, recognizing specific DNA sequences and activating immune cells to fight off infections. Dysregulation of TLR9 has been linked to autoimmune diseases, inflammatory conditions, and infectious diseases, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human TLR9 ELISA Kit, researchers can confidently measure TLR9 levels in human samples, advancing their understanding of immune responses and disease mechanisms. Get reliable results with this efficient and user-friendly ELISA kit from Assay Genie.
Product Name:
Human TLR9 (Toll-Like Receptor 9) ELISA Kit
SKU:
HUES02075
Target:
Human TLR9 (Toll-Like Receptor 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLR9. You can calculate the concentration of Human TLR9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
92-106
85-97
Average (%)
99
99
91
1:4
Range (%)
93-105
80-94
84-98
Average (%)
98
86
91
1:8
Range (%)
87-103
79-93
81-93
Average (%)
95
85
88
1:16
Range (%)
88-98
82-93
81-95
Average (%)
93
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.23
4.38
0.51
1.24
4.15
Standard deviation
0.03
0.07
0.2
0.03
0.06
0.18
C V (%)
6.12
5.69
4.57
5.88
4.84
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TLR9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR9 in samples. No significant cross-reactivity or interference between Human TLR9 and analogues was observed.
