The Human TM (Thrombin) ELISA Kit is specifically developed to detect levels of thrombin in human biological samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and consistent results for various research applications. Thrombin is a key enzyme involved in blood clotting and plays a crucial role in the coagulation cascade. Dysregulation of thrombin levels can lead to various blood disorders and cardiovascular diseases.
Therefore, studying thrombin levels can provide valuable insights into the mechanisms underlying these conditions and aid in the development of therapeutic interventions. With its reliable performance and ease of use, the Human TM (Thrombin) ELISA Kit is essential for researchers looking to investigate the role of thrombin in health and disease. Order your kit today from AssayGenie and accelerate your research.
Product Name:
Human TM (Thrombin) ELISA Kit
SKU:
HUES02757
Target:
Human TM (Thrombin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TM. You can calculate the concentration of Human TM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
90-103
90-104
Average (%)
97
96
97
1:4
Range (%)
88-100
83-95
89-102
Average (%)
95
90
94
1:8
Range (%)
92-107
80-90
88-99
Average (%)
100
85
93
1:16
Range (%)
91-104
83-97
80-93
Average (%)
96
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.87
5.64
16.71
1.88
6.16
15.2
Standard deviation
0.12
0.3
0.82
0.13
0.35
0.6
C V (%)
6.42
5.32
4.91
6.91
5.68
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TM in samples. No significant cross-reactivity or interference between Human TM and analogues was observed.
