The Human TM (Thrombomodulin) CLIA Kit is specifically designed for the quantitative measurement of Thrombomodulin levels in human plasma samples. This kit boasts high sensitivity and precision, allowing for accurate and reproducible results for research purposes.Thrombomodulin is a key protein involved in regulating blood coagulation and inflammation. It is essential for maintaining vascular integrity and preventing thrombosis.
Abnormal levels of Thrombomodulin have been linked to various diseases such as sepsis, thrombotic disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions.With its reliable performance and ease of use, the Human TM CLIA Kit is an essential tool for researchers and clinicians looking to study Thrombomodulin levels and its implications in various disease processes.
Product Name:
Human TM (Thrombomodulin) CLIA Kit
SKU:
HUES00143
Target:
Human TM (Thrombomodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TM. You can calculate the concentration of Human TM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
85-98
93-105
Average (%)
108
91
98
1:4
Range (%)
102-119
97-110
92-109
Average (%)
109
102
100
1:8
Range (%)
96-113
92-106
84-96
Average (%)
104
98
90
1:16
Range (%)
94-106
90-102
97-115
Average (%)
100
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.37
102.84
387.49
50.26
109.78
366.01
Standard deviation
3.96
8.64
33.98
5.71
9.22
28.04
C V (%)
8.19
8.4
8.77
11.36
8.4
7.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TM in samples. No significant cross-reactivity or interference between Human TM and analogues was observed.
