The Human TM (Thrombomodulin) ELISA Kit is specially designed to detect and quantify thrombomodulin levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research needs.Thrombomodulin is a vital protein involved in regulating blood clotting and inflammation, as well as maintaining vascular health. Dysregulation of thrombomodulin has been linked to various diseases, including thrombosis, sepsis, and cardiovascular disorders, making it a valuable biomarker for investigating these conditions and developing potential treatments.
Get reliable and insightful data on thrombomodulin levels with the Human TM (Thrombomodulin) ELISA Kit, an indispensable tool for researchers in the fields of cardiovascular health, hemostasis, and inflammatory diseases. Unlock new insights and drive discoveries with this cutting-edge assay kit.
Product Name:
Human TM (Thrombomodulin) ELISA Kit
SKU:
HUES01436
Target:
Human TM (Thrombomodulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TM. You can calculate the concentration of Human TM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
85-99
91-106
Average (%)
100
92
97
1:4
Range (%)
91-102
86-99
89-100
Average (%)
97
92
94
1:8
Range (%)
89-101
84-96
83-99
Average (%)
96
91
90
1:16
Range (%)
88-99
80-89
85-97
Average (%)
93
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.73
492.5
1427.15
225.03
477.96
1436.45
Standard deviation
11.62
22.51
74.21
15.12
20.03
65.07
C V (%)
5.24
4.57
5.2
6.72
4.19
4.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TM in samples. No significant cross-reactivity or interference between Human TM and analogues was observed.
