Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit
The Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit is a reliable and accurate tool for detecting TMPRSS2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TMPRSS2 is a transmembrane protease that plays a crucial role in various biological processes, including viral entry into host cells and cell signaling pathways. Dysregulation of TMPRSS2 has been associated with conditions such as prostate cancer and viral infections, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With its advanced technology and user-friendly design, the Human TMPRSS2 CLIA Kit is an essential tool for researchers and clinicians conducting studies on TMPRSS2-related pathways and diseases. Invest in this kit today to advance your research and make significant contributions to the field of biomedicine.
Product Name:
Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit
SKU:
HUES00807
Target:
Human TMPRSS2 (Transmembrane Protease, Serine 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TMPRSS2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TMPRSS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TMPRSS2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TMPRSS2. You can calculate the concentration of Human TMPRSS2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
101-118
93-108
Average (%)
101
107
101
1:4
Range (%)
90-102
85-97
97-113
Average (%)
96
91
104
1:8
Range (%)
91-107
84-96
87-101
Average (%)
98
91
94
1:16
Range (%)
91-104
90-104
89-105
Average (%)
96
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.73
264.75
807.97
97.98
244.44
824.8
Standard deviation
10.68
20.81
80.96
12.57
18.55
54.11
C V (%)
10.5
7.86
10.02
12.83
7.59
6.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TMPRSS2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TMPRSS2 in samples. No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed.
