Human TNF Receptor Associated Factor 5 (TRAF5) ELISA Kit
The Human TNF Receptor-Associated Factor 5 (TRAF5) ELISA Kit is specifically designed for the accurate quantification of TNF receptor-associated factor 5 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TNF receptor-associated factor 5 is a crucial signaling protein involved in the regulation of immune responses and inflammation. It plays a significant role in various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potentially developing novel therapies.
With its advanced technology and robust performance, the Human TRAF5 ELISA Kit is a valuable tool for researchers and clinicians looking to explore the role of TNF receptor-associated factor 5 in disease pathogenesis and progression. Trust in the accuracy and precision of this kit to accelerate your research and discovery process.
Product Name:
Human TNF Receptor Associated Factor 5 (TRAF5) ELISA Kit
Product Code:
HUDL02983
Size:
96 Assays
Target:
TRAF5
Synonyms:
RNF84, RING finger protein 84
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
