Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
The Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily Member 1A) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TNFRSF1A levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of TNFRSF1A in various diseases and conditions.TNFRSF1A is a critical receptor involved in the tumor necrosis factor signaling pathway, regulating inflammation, cell survival, and apoptosis. Dysregulation of TNFRSF1A has been linked to various autoimmune diseases, inflammatory disorders, and cancer.
Therefore, accurate measurement of TNFRSF1A levels is crucial for understanding its role in disease pathogenesis and developing targeted therapies.With its high sensitivity and specificity, the Human TNFRSF1A CLIA Kit from AssayGenie is an indispensable tool for researchers looking to advance their understanding of TNFRSF1A biology and its implications in health and disease. Get reliable and reproducible results with this advanced assay kit.
Product Name:
Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
SKU:
HUES00176
Target:
Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF1A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNFRSF1A. You can calculate the concentration of Human TNFRSF1A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
88-100
93-107
Average (%)
95
94
99
1:4
Range (%)
95-109
101-119
93-104
Average (%)
100
108
98
1:8
Range (%)
90-103
85-100
90-107
Average (%)
95
92
97
1:16
Range (%)
97-114
100-113
96-111
Average (%)
105
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.34
49.72
194.34
25.21
51.53
194.25
Standard deviation
3.15
3.7
16.56
2.08
5.4
15.64
C V (%)
12.94
7.44
8.52
8.25
10.48
8.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNFRSF1A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
