Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit
The Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily Member 1A) ELISA Kit is a cutting-edge tool for the precise measurement of TNFRSF1A levels in human biological samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an indispensable resource for various research endeavors.TNFRSF1A is a pivotal receptor protein involved in the regulation of immune responses and inflammatory processes. Dysregulation of TNFRSF1A signaling is implicated in autoimmune diseases, inflammatory disorders, and cancer progression.
By quantifying TNFRSF1A levels, researchers can gain valuable insights into disease mechanisms and explore potential therapeutic interventions.Key Features:- Detects TNFRSF1A levels in human biological samples- High sensitivity and specificity for reliable results- Facilitates research on autoimmune diseases, inflammatory disorders, and cancer- Essential tool for biomarker analysis and drug development studiesInvest in the Human TNFRSF1A ELISA Kit for a comprehensive analysis of TNFRSF1A levels and accelerate your research in immunology, oncology, and inflammation-related fields.
Product Name:
Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit
SKU:
HUES01474
Target:
Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF1A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFRSF1A. You can calculate the concentration of Human TNFRSF1A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
93-106
91-106
Average (%)
102
99
97
1:4
Range (%)
90-105
83-94
85-100
Average (%)
98
89
92
1:8
Range (%)
91-105
85-96
87-97
Average (%)
96
90
92
1:16
Range (%)
91-105
83-94
83-95
Average (%)
99
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.21
74.91
187.9
20.91
67.85
199.93
Standard deviation
1.27
3.03
7.27
1.4
4.05
10.96
C V (%)
5.47
4.04
3.87
6.7
5.97
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNFRSF1A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
