Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit
The Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily Member 1B) CLIA Kit is a powerful tool for detecting TNFRSF1B levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.TNFRSF1B is a critical receptor involved in the tumor necrosis factor signaling pathway, playing a key role in regulating inflammation, apoptosis, and immune response. Dysregulation of TNFRSF1B has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
Therefore, measuring TNFRSF1B levels can provide valuable insights into disease progression and therapeutic targets.With its advanced technology and robust performance, the Human TNFRSF1B CLIA Kit from Assay Genie is a valuable asset for researchers studying the role of TNFRSF1B in health and disease. Obtain accurate and reproducible data to advance your research efforts with this innovative kit.
Product Name:
Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit
SKU:
HUES01274
Target:
Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF1B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNFRSF1B. You can calculate the concentration of Human TNFRSF1B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
91-102
101-118
Average (%)
104
97
109
1:4
Range (%)
101-116
87-101
87-102
Average (%)
108
94
93
1:8
Range (%)
101-115
91-107
101-118
Average (%)
108
98
108
1:16
Range (%)
89-101
96-107
85-99
Average (%)
95
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
108
EDTA plasma (n=5)
91-107
97
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.8
56.79
202.7
26.24
55.21
199.08
Standard deviation
2.32
4.83
16.72
2.51
4.42
12.96
C V (%)
8.66
8.51
8.25
9.57
8.01
6.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNFRSF1B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFRSF1B in samples. No significant cross-reactivity or interference between Human TNFRSF1B and analogues was observed.
