The Human TNNC2 (Troponin C type 2) CLIA Kit is specifically designed for the accurate and sensitive detection of TNNC2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.TNNC2 is a critical protein that plays a key role in muscle contraction and function. As a member of the troponin complex, it is essential for regulating muscle contraction by binding to calcium ions.
Dysregulation of TNNC2 expression has been linked to cardiac diseases, making it a valuable biomarker for studying such conditions and developing potential therapeutic interventions.Overall, the Human TNNC2 CLIA Kit provides researchers with a valuable tool for studying TNNC2 levels in human samples, offering insights into its role in muscle function and its potential implications in cardiac diseases.
Product Name:
Human TNNC2 (Troponin C Type 2) CLIA Kit
SKU:
HUES00810
Target:
Human TNNC2 (Troponin C Type 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNNC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNNC2. You can calculate the concentration of Human TNNC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
100-118
101-117
Average (%)
97
108
109
1:4
Range (%)
93-108
90-104
99-117
Average (%)
98
96
107
1:8
Range (%)
92-105
90-103
88-99
Average (%)
97
96
93
1:16
Range (%)
93-110
88-104
96-109
Average (%)
101
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.79
104.67
368.92
34.67
107.33
356.12
Standard deviation
4.09
12.36
29.4
3.06
8.25
22.15
C V (%)
11.12
11.81
7.97
8.83
7.69
6.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNNC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNC2 in samples. No significant cross-reactivity or interference between Human TNNC2 and analogues was observed.
