Human TNNT1 (Troponin T Type 1, Slow Skeletal) CLIA Kit
The Human TNNT1 (Troponin T Type 1 Slow Skeletal) CLIA Kit is a cutting-edge assay designed for the precise measurement of TNNT1 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.TNNT1 is a key protein involved in muscle contraction, particularly in slow-twitch skeletal muscle fibers.
Mutations in TNNT1 have been linked to various muscle disorders, making it an important biomarker for studying muscle function and disease. The Human TNNT1 CLIA Kit provides researchers with a powerful tool for investigating these conditions and developing potential treatments.
Product Name:
Human TNNT1 (Troponin T Type 1, Slow Skeletal) CLIA Kit
SKU:
HUES00857
Target:
Human TNNT1 (Troponin T Type 1, Slow Skeletal)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNNT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNNT1. You can calculate the concentration of Human TNNT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
98-109
93-108
Average (%)
106
103
100
1:4
Range (%)
86-99
87-98
96-109
Average (%)
91
93
104
1:8
Range (%)
93-106
102-116
85-98
Average (%)
99
108
91
1:16
Range (%)
103-115
89-101
96-112
Average (%)
109
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
99-116
106
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.6
53.79
166.94
28.19
50.86
174.29
Standard deviation
3.07
3.79
12.89
3.33
4.87
15.22
C V (%)
11.12
7.05
7.72
11.81
9.58
8.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNNT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
