The Human TNPO1 (Transportin-1) CLIA Kit is specially designed for the precise quantification of Transportin-1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for a wide range of research applications.Transportin-1, also known as TNPO1, is a key protein involved in nucleocytoplasmic transport, playing a crucial role in the transportation of various molecules including proteins and RNA.
Dysregulation of TNPO1 has been implicated in several diseases such as cancer, viral infections, and neurodegenerative disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.This Human TNPO1 CLIA Kit provides researchers with a reliable tool to study the role of Transportin-1 in various physiological and pathological processes, offering valuable insights into disease mechanisms and potential therapeutic interventions.
Product Name:
Human TNPO1 (Transportin 1) CLIA Kit
SKU:
HUES01215
Target:
Human TNPO1 (Transportin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNPO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNPO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNPO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNPO1. You can calculate the concentration of Human TNPO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
97-113
95-106
Average (%)
102
105
100
1:4
Range (%)
94-106
97-113
97-107
Average (%)
99
103
102
1:8
Range (%)
91-102
96-114
94-108
Average (%)
96
104
100
1:16
Range (%)
90-104
95-107
89-101
Average (%)
96
100
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
99
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.85
4.8
19.99
1.91
4.47
20.37
Standard deviation
0.18
0.53
1.58
0.23
0.47
1.3
C V (%)
9.73
11.04
7.9
12.04
10.51
6.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNPO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNPO1 in samples. No significant cross-reactivity or interference between Human TNPO1 and analogues was observed.
