Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) ELISA Kit
The Human Toll/Interleukin-1 Receptor Domain-Containing Adaptor Protein (TIRAP) ELISA Kit offered by Assay Genie is a cutting-edge tool for the precise quantification of TIRAP levels in human samples. TIRAP is a key adaptor protein involved in Toll-like receptor signaling, playing a crucial role in the immune response to pathogen invasion and inflammatory processes.This ELISA kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs.
It is suitable for the analysis of serum, plasma, and cell culture supernatants, making it a versatile option for a wide range of experimental settings.By accurately measuring TIRAP levels, researchers can gain valuable insights into the mechanisms underlying various diseases, including infections, autoimmune disorders, and inflammatory conditions. This kit is a valuable asset for investigating the role of TIRAP in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) ELISA Kit
Product Code:
HUDL02902
Size:
96 Assays
Target:
TIRAP
Synonyms:
Mal, Wyatt, MyD88 Adapter-Like, Adaptor protein Wyatt
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
