Description
Product Name: | Human Toxoplasma IgM ELISA Kit |
SKU: | HDES0021 |
Size: | 96T |
Target: | TOX-IgM |
Detection Method: | Capture ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.8%, Specificity 99.4% |
Sample Type: | Serum, Plasma |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Capture-ELISA as the method to detect the TOX-IgM in human serum or plasma. The ELISA Microtiter plate provided in this kit has been pre-coated with Mouse-anti-human IgM (μ chain). Samples are added to the ELISA Microtiter plate wells and the IgM antibody in which will be captured. Free components are washed away (including the specific IgG antibody). HRP conjugated TOX antigen is added to each well and react with the compound to form “Mouse-anti-human IgM (μ chain)-TOX IgM - TOX antigen-HRP” compound. The TMB substrate is added after washing to initiate the color developing reaction. The presence of TOX-IgM can be determined according to the OD value after colorimetric assay with the Micro-plate Reader.