The Human TP-TXA2R (Thromboxane Receptor) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TP-TXA2R levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for a wide range of research applications.TP-TXA2R is a key receptor involved in the thromboxane signaling pathway, playing a crucial role in various physiological processes including platelet aggregation, vasoconstriction, and inflammation.
Dysregulation of this receptor has been implicated in cardiovascular diseases, inflammatory disorders, and cancer, making it a promising biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human TP-TXA2R CLIA Kit offers researchers a convenient and accurate method for quantifying TP-TXA2R levels in human samples, providing valuable insights into the role of this receptor in health and disease.
Product Name:
Human TP/TXA2R (Thromboxane Receptor) CLIA Kit
SKU:
HUES00835
Target:
Human TP/TXA2R (Thromboxane Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TP/TXA2R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP/TXA2R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP/TXA2R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TP/TXA2R. You can calculate the concentration of Human TP/TXA2R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
86-98
90-105
Average (%)
91
91
97
1:4
Range (%)
94-109
85-96
97-109
Average (%)
101
91
103
1:8
Range (%)
88-102
87-101
85-97
Average (%)
94
94
92
1:16
Range (%)
97-113
91-105
91-104
Average (%)
104
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.63
250.41
869.33
97.43
229.9
891.1
Standard deviation
10.79
29.75
73.63
11.57
17.04
54.36
C V (%)
10.41
11.88
8.47
11.88
7.41
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TP/TXA2R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TP/TXA2R in samples. No significant cross-reactivity or interference between Human TP/TXA2R and analogues was observed.
