The Human TP63 (Tumor Protein p63) CLIA Kit is specifically designed for the precise quantification of TP63 levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures consistent and accurate results, making it an excellent choice for a variety of research purposes.TP63 is a critical protein that plays a role in cellular development and proliferation, particularly in cancer research.
Elevated TP63 levels have been associated with various cancers, making it a valuable biomarker for studying cancer progression and developing targeted therapies.Get reliable and reproducible results with the Human TP63 CLIA Kit, and delve deeper into the role of TP63 in cancer and other diseases. Visit the provided URL for more information and to order the kit for your research needs.
Product Name:
Human TP63 (Tumor Protein p63) CLIA Kit
SKU:
HUES01197
Target:
Human TP63 (Tumor Protein p63)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TP63. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP63 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP63, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TP63. You can calculate the concentration of Human TP63 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-100
100-116
Average (%)
93
94
106
1:4
Range (%)
94-110
87-101
91-102
Average (%)
101
92
96
1:8
Range (%)
97-110
85-98
85-97
Average (%)
102
90
91
1:16
Range (%)
86-98
99-117
85-96
Average (%)
92
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
101-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.88
281.05
954.41
97.33
298.16
1028.76
Standard deviation
8.74
20.71
83.03
9.84
21.86
64.4
C V (%)
9.02
7.37
8.7
10.11
7.33
6.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TP63 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TP63 in samples. No significant cross-reactivity or interference between Human TP63 and analogues was observed.
