Human TPS (Tissue Polypeptide Specific Antigen) CLIA Kit
The Human TPS (Tissue Polypeptide-Specific Antigen) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TPS levels in human serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it a valuable tool for researchers studying various diseases and conditions.TPS is a tissue-specific antigen that is often elevated in patients with certain types of cancer, particularly in those with liver, pancreatic, and lung cancers.
Monitoring TPS levels can aid in disease diagnosis, prognosis, and treatment response assessment.This CLIA kit offers a simple and efficient method for detecting TPS, allowing for high throughput testing and precise quantification of antigen levels. Whether investigating cancer biomarkers or studying disease mechanisms, the Human TPS CLIA Kit provides researchers with a reliable and accurate tool for their studies.
Product Name:
Human TPS (Tissue Polypeptide Specific Antigen) CLIA Kit
SKU:
HUES01219
Target:
Human TPS (Tissue Polypeptide Specific Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TPS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TPS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TPS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TPS. You can calculate the concentration of Human TPS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
87-102
90-105
Average (%)
104
93
98
1:4
Range (%)
102-120
86-98
97-111
Average (%)
109
91
102
1:8
Range (%)
88-99
99-115
91-103
Average (%)
94
107
97
1:16
Range (%)
92-106
86-99
95-109
Average (%)
98
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.03
296.85
855.77
93.73
312.77
936.71
Standard deviation
8.22
26.24
69.83
10.52
33.15
61.54
C V (%)
8.74
8.84
8.16
11.22
10.6
6.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TPS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TPS in samples. No significant cross-reactivity or interference between Human TPS and analogues was observed.
