Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
The Human TRAIL (TNFSF10) CLIA Kit is a cutting-edge assay designed for the quantitative measurement of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) levels in human samples. This kit is highly sensitive and specific, providing accurate and reproducible results for serum, plasma, and cell culture supernatants.TRAIL is a key regulator of apoptosis, inducing programmed cell death in cancer cells while sparing normal cells.
Dysregulation of TRAIL signaling pathways has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases. The Human TRAIL CLIA Kit offers researchers a valuable tool for studying the role of TRAIL in disease pathogenesis and identifying potential therapeutic targets.
Product Name:
Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
SKU:
HUES00907
Target:
Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TRAIL/TNFSF10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TRAIL/TNFSF10. You can calculate the concentration of Human TRAIL/TNFSF10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
100-119
100-116
Average (%)
95
108
107
1:4
Range (%)
98-113
85-98
98-114
Average (%)
104
90
105
1:8
Range (%)
98-111
86-98
93-107
Average (%)
103
93
98
1:16
Range (%)
101-117
97-112
102-118
Average (%)
109
103
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.76
79.38
247.65
25.13
83.06
237.53
Standard deviation
2.74
8.76
16.0
2.7
6.65
26.94
C V (%)
10.64
11.04
6.46
10.74
8.01
11.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TRAIL/TNFSF10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
