Human Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
The Human Transforming Growth Factor Beta 3 (TGFB3) ELISA Kit is specifically designed for the accurate and precise detection of TGFB3 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.TGFB3 is a key growth factor involved in various cellular processes, including cell growth, proliferation, differentiation, and apoptosis.
It plays a critical role in regulating immune responses, wound healing, and tissue repair, making it a valuable biomarker for studying diseases such as cancer, fibrosis, and autoimmune disorders.With the Human TGFB3 ELISA Kit, researchers can confidently investigate the role of TGFB3 in different biological pathways and diseases, paving the way for potential therapeutic interventions and personalized medicine approaches.
Product Name:
Human Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
Product Code:
HUDL02870
Size:
96 Assays
Target:
TGFb3
Synonyms:
TGF-B3, LAP, Latency-associated peptide
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
