The Human Transglutaminase 2 (Tissue TGm2) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of TGm2 levels in human tissue samples. This kit is ideal for researchers studying the role of TGm2 in various diseases and conditions, including autoimmune disorders, tissue fibrosis, and neurodegenerative diseases.Transglutaminase 2 is an important enzyme involved in tissue repair and remodeling, as well as in the pathogenesis of various diseases. Elevated levels of TGm2 have been associated with conditions such as celiac disease and certain types of cancer.
By accurately measuring TGm2 levels in tissue samples, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Human Transglutaminase 2 (Tissue TGm2) ELISA Kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs. With its user-friendly protocol and quick assay time, this kit is a valuable tool for researchers looking to study the role of TGm2 in health and disease.
Product Name:
Human Transglutaminase 2, Tissue (TGM2) ELISA Kit
Product Code:
HUDL02876
Size:
96 Assays
Target:
TGM2
Synonyms:
tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
