Human Transient Receptor Potential Cation Channel Subfamily M, Member 1 (TRPM1) ELISA Kit
The Human Transient Receptor Potential Cation Channel Subfamily M Member 1 (TRPM1) ELISA Kit is a powerful tool for the precise measurement of TRPM1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an essential asset for a wide variety of research studies.TRPM1 is a key protein involved in sensory signaling and ion transport in the human body.
Dysfunction of TRPM1 has been linked to various health conditions including visual disorders, skin pigmentation abnormalities, and neurological disorders. Studying TRPM1 levels can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of potential therapeutic interventions.Order the Human TRPM1 ELISA Kit today and unlock the potential for groundbreaking research in the field of ion channel biology and human health.
Product Name:
Human Transient Receptor Potential Cation Channel Subfamily M, Member 1 (TRPM1) ELISA Kit
Product Code:
HUDL02996
Size:
96 Assays
Target:
TRPM1
Synonyms:
LTRPC1, MLSN1, Melastatin 1, Long transient receptor potential channel 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
