Human Transmembrane Protease,Serine 6 (TMPRSS6) ELISA Kit
The Human Transmembrane Protease, Serine 6 (TMPRSS6) ELISA Kit is specifically designed for the accurate detection of TMPRSS6 levels in human serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit ensures dependable and reproducible results, making it an excellent choice for a variety of research applications.TMPRSS6 is a key transmembrane protease involved in iron homeostasis and regulation of hepcidin, a hormone that plays a crucial role in iron metabolism.
Dysregulation of TMPRSS6 has been linked to iron-related disorders such as iron-deficiency anemia and hemochromatosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human TMPRSS6 ELISA Kit offers researchers a reliable tool for quantifying TMPRSS6 levels and studying its role in iron metabolism and related disorders.
Product Name:
Human Transmembrane Protease,Serine 6 (TMPRSS6) ELISA Kit
Product Code:
HUDL02927
Size:
96 Assays
Target:
TMPRSS6
Synonyms:
Matriptase-2
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
