Human TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) CLIA Kit
The Human TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of TREM-1 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a wide variety of research applications.TREM-1 is a key receptor expressed on myeloid cells that plays a crucial role in inflammatory responses and immune regulation.
Dysregulation of TREM-1 has been linked to various diseases including sepsis, inflammatory bowel disease, and arthritis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Human TREM-1 CLIA Kit from Assay Genie is a valuable tool for researchers and clinicians alike looking to gain deeper insights into the role of TREM-1 in health and disease.
Product Name:
Human TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) CLIA Kit
SKU:
HUES00908
Target:
Human TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TREM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TREM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TREM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TREM-1. You can calculate the concentration of Human TREM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
91-104
99-115
Average (%)
103
99
105
1:4
Range (%)
87-97
87-100
89-100
Average (%)
92
94
94
1:8
Range (%)
92-102
96-109
101-116
Average (%)
97
103
107
1:16
Range (%)
103-121
101-117
88-101
Average (%)
110
108
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.77
124.86
399.46
53.76
136.05
385.49
Standard deviation
6.53
12.34
35.35
5.13
16.26
28.72
C V (%)
12.61
9.88
8.85
9.54
11.95
7.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TREM-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TREM-1 in samples. No significant cross-reactivity or interference between Human TREM-1 and analogues was observed.
