Description
Product Name: | Human Treponema Pallidum (TP) Antibody ELISA Kit |
SKU: | HDES0037 |
Size: | 96T |
Target: | TP-Ab |
Detection Method: | Sandwich ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.1%, Specificity 100% |
Sample Type: | Serum, Plasma |
Assay Time: | 2 h |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Sandwich-ELISA as the method to detect the Treponema Pallidum (TP) antibody in human serum or plasma. The ELISA Microtiter plate provided in this kit has been pre-coated with recombinant TP antigen. Samples are added to the ELISA Microtiter plate wells and the TP antibody in the samples will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugated recombinant TP antigen is added to each well and react with the compound to form “TP antigen- TP antibody-HRP conjugated TP antigen” compound. The TMB substrate is added to initiate the color developing reaction. The presence of TP antibody can be determined according to the OD value after colorimetric assay with the Micro-plate Reader.