Human Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit
Based on the information provided in the URL, a product description for the Human Troponin I Type 1 Slow Skeletal (TNNI1) ELISA Kit could look like this:The Human Troponin I Type 1 Slow Skeletal (TNNI1) ELISA Kit is specifically designed for the precise detection of TNNI1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research purposes.TNNI1 is a critical protein involved in muscle function, particularly in skeletal muscle, influencing muscle contraction and exercise performance.
It serves as a valuable biomarker for conditions impacting skeletal muscle health, such as muscular dystrophy, heart failure, and skeletal muscle injuries, making it a crucial tool for investigating these conditions and exploring potential therapeutic strategies.Overall, the Human Troponin I Type 1 Slow Skeletal (TNNI1) ELISA Kit provides researchers with a powerful tool for studying skeletal muscle function and health, with the potential to advance our understanding of various muscular disorders and inform the development of targeted treatments.
Product Name:
Human Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit
Product Code:
HUDL02949
Size:
96 Assays
Target:
TNNI1
Synonyms:
TNN1, Troponin I, slow-twitch isoform
Detection Method:
Sandwich
Range:
46.875-3,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
