The Human TRX (Thioredoxin) CLIA Kit is specifically designed for the precise measurement of thioredoxin levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures trustworthy and consistent results, making it perfect for various research applications.Thioredoxin is a key protein involved in redox regulation, playing a crucial role in maintaining cellular homeostasis and protecting cells from oxidative stress.
Its dysregulation has been linked to various diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Learn more about the Human TRX (Thioredoxin) CLIA Kit at www.assaygenie.com/human-trx-thioredoxin-clia-kit-hues00965/
Product Name:
Human Trx (Thioredoxin) CLIA Kit
SKU:
HUES00965
Target:
Human Trx (Thioredoxin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Trx. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Trx and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Trx, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Trx. You can calculate the concentration of Human Trx in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
99-112
95-108
Average (%)
105
104
100
1:4
Range (%)
92-106
96-113
89-104
Average (%)
98
103
95
1:8
Range (%)
94-108
90-101
100-115
Average (%)
101
96
107
1:16
Range (%)
89-99
90-104
93-105
Average (%)
94
97
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.2
550.39
1356.34
201.82
506.12
1447.54
Standard deviation
18.6
40.95
115.7
21.31
55.88
91.92
C V (%)
9.48
7.44
8.53
10.56
11.04
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Trx concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
