The Human TRXR1 (Thioredoxin Reductase 1) CLIA Kit is a reliable and precise tool for the quantitative measurement of TRXR1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TRXR1 is a key enzyme involved in redox homeostasis, playing a critical role in cellular antioxidant defense and regulating various cellular processes. Dysregulation of TRXR1 has been implicated in a range of diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
With its superior performance and ease of use, the Human TRXR1 CLIA Kit is an indispensable tool for researchers seeking to explore the role of TRXR1 in health and disease, paving the way for advances in personalized medicine and targeted therapies.
Product Name:
Human TrxR1 (Thioredoxin Reductase 1) CLIA Kit
SKU:
HUES00966
Target:
Human TrxR1 (Thioredoxin Reductase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TrxR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TrxR1. You can calculate the concentration of Human TrxR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
87-100
88-99
Average (%)
99
92
93
1:4
Range (%)
87-100
98-114
96-113
Average (%)
94
106
104
1:8
Range (%)
95-111
94-107
96-111
Average (%)
102
101
104
1:16
Range (%)
96-109
92-106
99-114
Average (%)
101
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
96
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.97
300.95
929.28
99.8
279.57
841.33
Standard deviation
11.9
35.78
77.32
9.01
21.47
85.56
C V (%)
12.53
11.89
8.32
9.03
7.68
10.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TrxR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
