The Human TRXR1 (Thioredoxin Reductase 1) ELISA Kit is a powerful tool for the precise measurement of TRXR1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an excellent choice for a wide array of research studies.TRXR1 is an essential enzyme that plays a crucial role in the maintenance of cellular redox homeostasis and the regulation of oxidative stress. Dysregulation of TRXR1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, the accurate measurement of TRXR1 levels is vital for understanding its role in disease pathogenesis and for the development of potential therapeutic interventions.Overall, the Human TRXR1 ELISA Kit provides researchers with a reliable and efficient tool for studying the role of TRXR1 in health and disease, offering valuable insights into potential diagnostic and therapeutic strategies.
Product Name:
Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit
SKU:
HUES02716
Target:
Human TrxR1 (Thioredoxin Reductase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TrxR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TrxR1. You can calculate the concentration of Human TrxR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
86-99
91-105
Average (%)
99
92
98
1:4
Range (%)
93-109
84-95
82-96
Average (%)
100
90
89
1:8
Range (%)
88-100
81-92
90-101
Average (%)
93
87
95
1:16
Range (%)
87-98
79-91
86-98
Average (%)
92
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.42
4.77
0.44
1.48
5.18
Standard deviation
0.02
0.06
0.23
0.03
0.09
0.21
C V (%)
4.35
4.23
4.82
6.82
6.08
4.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TrxR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
