The Human TSP-4 (Thrombospondin-4) ELISA Kit is specially designed for the precise measurement of thrombospondin-4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.Thrombospondin-4 is a key protein involved in various biological processes, including cell adhesion, migration, and tissue remodeling.
It is also implicated in conditions such as cardiovascular diseases, arthritis, and cancer, making it a valuable biomarker for studying these diseases and potential treatment options.With its reliable performance and user-friendly design, the Human TSP-4 ELISA Kit is a valuable tool for researchers seeking to explore the role of thrombospondin-4 in health and disease.
Product Name:
Human TSP-4 (Thrombospondin-4) ELISA Kit
SKU:
HUES02731
Target:
Human TSP-4 (Thrombospondin-4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TSP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TSP-4. You can calculate the concentration of Human TSP-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
95-109
91-104
Average (%)
103
100
96
1:4
Range (%)
89-102
84-95
86-97
Average (%)
94
90
92
1:8
Range (%)
88-100
87-100
82-97
Average (%)
93
93
89
1:16
Range (%)
93-103
80-92
87-103
Average (%)
98
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.37
4.95
0.46
1.41
5.19
Standard deviation
0.03
0.07
0.21
0.03
0.07
0.26
C V (%)
5.88
5.11
4.24
6.52
4.96
5.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TSP-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSP-4 in samples. No significant cross-reactivity or interference between Human TSP-4 and analogues was observed.
Vera Dobelmann et al.
Thrombospondin-4 as potential cerebrospinal fluid biomarker for therapy response in pediatric spinal muscular atrophy
