The Human TTN (Titin) CLIA Kit is specifically designed for the precise measurement of titin levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for various research purposes.Titin is a vital protein that plays a key role in muscle contraction and sarcomere structure.
Abnormal levels of titin have been associated with various muscular and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.For researchers looking to investigate the role of titin in health and disease, the Human TTN (Titin) CLIA Kit is an indispensable tool that provides accurate and reliable data for their studies.
Product Name:
Human TTN (Titin) CLIA Kit
SKU:
HUES00316
Target:
Human TTN (Titin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TTN. You can calculate the concentration of Human TTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
86-99
97-111
Average (%)
100
92
103
1:4
Range (%)
100-115
91-106
97-113
Average (%)
107
98
105
1:8
Range (%)
101-115
101-117
87-102
Average (%)
107
110
94
1:16
Range (%)
103-115
100-114
96-112
Average (%)
109
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.41
499.22
1421.58
199.23
467.19
1515.25
Standard deviation
20.7
36.69
146.99
22.75
33.54
153.04
C V (%)
10.87
7.35
10.34
11.42
7.18
10.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TTN in samples. No significant cross-reactivity or interference between Human TTN and analogues was observed.
