The Human TTN (Titin) ELISA Kit is specifically designed to detect and quantify titin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable tool for researchers in various fields.Titin is the largest known protein in the human body and plays a critical role in muscle contraction and relaxation. Abnormalities in titin expression have been implicated in various muscular disorders and cardiac diseases, making it a significant biomarker for studying these conditions and potential therapeutic interventions.
By using the Human TTN ELISA Kit, researchers can gain valuable insights into the role of titin in health and disease, ultimately contributing to advancements in the understanding and treatment of musculoskeletal and cardiovascular disorders.
Product Name:
Human TTN (Titin) ELISA Kit
SKU:
HUES01651
Target:
Human TTN (Titin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TTN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TTN. You can calculate the concentration of Human TTN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
92-104
99-115
Average (%)
99
97
106
1:4
Range (%)
93-108
80-93
86-101
Average (%)
99
86
92
1:8
Range (%)
92-105
81-92
87-103
Average (%)
97
87
94
1:16
Range (%)
88-99
85-96
87-102
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
100
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.13
4.55
0.51
1.13
4.84
Standard deviation
0.03
0.06
0.22
0.04
0.06
0.25
C V (%)
6.52
5.31
4.84
7.84
5.31
5.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TTN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TTN in samples. No significant cross-reactivity or interference between Human TTN and analogues was observed.
