Human Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit
The Human Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12) ELISA Kit is specifically designed for the precise measurement of TNFSF12 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TNFSF12, also known as TWEAK (tumor necrosis factor-like weak inducer of apoptosis), is a key protein in the TNF superfamily that plays a critical role in regulating cell death, proliferation, and inflammation.
Dysregulation of TNFSF12 has been implicated in various diseases, including cancer, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.With its reliable performance and easy-to-use format, the Human TNFSF12 ELISA Kit is an essential tool for researchers studying the molecular mechanisms of disease and developing novel treatment strategies. Invest in this kit to unlock valuable insights into the role of TNFSF12 in human health and disease.
Product Name:
Human Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit
Product Code:
HUDL02938
Size:
96 Assays
Target:
TNFSF12
Synonyms:
APO3L, DR3LG, TWEAK, APO3 ligand, TNF-related weak inducer of apoptosis
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
