Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit
The Human Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9) ELISA Kit is a powerful tool for the precise measurement of TNFSF9 levels in human samples. TNFSF9, also known as 4-1BBL, is a member of the TNF superfamily and plays a crucial role in immune responses and inflammation.This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for researchers studying TNFSF9's involvement in diseases such as cancer, autoimmune disorders, and inflammatory conditions.
By accurately quantifying TNFSF9 levels in serum, plasma, and cell culture supernatants, this kit enables researchers to gain valuable insights into the role of TNFSF9 in disease pathogenesis and potential therapeutic targets.With its ease of use and high performance, the Human TNFSF9 ELISA Kit is an indispensable tool for advancing research in immunology, oncology, and inflammation. Get your kit today and unlock the potential of TNFSF9 research!
Product Name:
Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit
Product Code:
HUDL02943
Size:
96 Assays
Target:
TNFSF9
Synonyms:
4-1BB-L, Receptor 4-1BB Ligand, Homolog Of Mouse 4-1BB-L
Detection Method:
Sandwich
Range:
15.62-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
