Human Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit
The Human Tumor Necrosis Factor Receptor Superfamily Member 1A (TNFRSF1A) ELISA Kit is a powerful tool for the precise measurement of TNFRSF1A levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it perfect for a wide array of research applications.TNFRSF1A, also known as TNF receptor 1, is a key player in signal transduction pathways that regulate inflammation, apoptosis, and immune responses.
Dysregulation of TNFRSF1A has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and cancer. Thus, this ELISA kit is invaluable for studying TNFRSF1A function and developing targeted therapies for associated diseases.Don't miss out on the opportunity to enhance your research with the Human TNFRSF1A ELISA Kit. Visit our website to learn more and place your order today.
Product Name:
Human Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
