Human Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit
The Human Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4) ELISA Kit is a cutting-edge tool for the precise measurement of TNFRSF4 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable asset for various research endeavors.TNFRSF4 is a crucial receptor protein involved in signal transduction and immune regulation. Its dysregulation has been implicated in various diseases, including autoimmune disorders and cancer.
By accurately quantifying TNFRSF4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets, paving the way for improved diagnostic and treatment strategies.In summary, the Human TNFRSF4 ELISA Kit offers a reliable and efficient solution for studying TNFRSF4 biology and its implications in health and disease. Trust in this advanced kit to advance your research and enhance your understanding of TNFRSF4-related pathways.
Product Name:
Human Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
