Description
| Product Name: | Human TYRP1 Recombinant Protein |
| Product Code: | RPPB5056 |
| Size: | 5µg |
| Species: | Human |
| Target: | TYRP1 |
| Synonyms: | Tyrosinase Related Protein 1, Tyrosinase-Related Protein1, Melanoma Antigen Gp75, Glycoprotein 75, DHICA Oxidase, Catalase B, CAS2, TRP1,TYRP, TRP, 5,6-Dihydroxyindole-2-Carboxylic Acid Oxidase, EC 1.14.18.1, EC1.14.18.-, EC 1.14.18, B-PROTEIN, TRP-1, TYRRP, CATB, GP75, OCA3, 5,6-dihydroxyindole-2-carboxylicacid oxidase. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TYRP1 protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADLQFPRQCATVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHCNGNFSGHNCG TCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILGPDGNTPQFEN ISIYNYFVWT HYYSVKKTFLGVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNFATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCD SLEDYDTLGT LCNSTEDGPI RRNPAGNVARPMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAHLFLNGTGGQT HLSPNDPIFVLLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSR EFSVPEHHHHHH |
TYRP1,also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomalenzyme which is a member of the tyrosinase family and takes a significant partin the melanin biosynthetic pathway. TYRP1 is a melanocyte-specificgene which is involved in eumelanin synthesis. Furthermore, TYRP1 is implicatedin the oxidation of 5,6-dihydroxyindole-2-carboxylicacid-DHICA into indole-5,6-quinone-2-carboxylic acid. TYRP1 is regulated by themicrophthalmia-associated transcriptionfactor-MITF.
TYRP1 Human Recombinant produced in Sf9 Baculovirus cellsis a single, non-glycosylated polypeptide chain containing 462 amino acids (25-477a.a)and having a molecular mass of 52.5kDa. (Molecular size on SDS-PAGE will appearat approximately 50-70kDa).TYRP1 is fused to a 6 amino acid His-tag at C-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TYRP1: Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3); also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. Belongs to the tyrosinase family.Protein type: EC 1.14.18.-; Oxidoreductase; Amino Acid Metabolism - tyrosine; Membrane protein, integralChromosomal Location of Human Ortholog: 9p23Cellular Component: melanosome membrane; integral to membrane; melanosome; endosome membraneMolecular Function: protein binding; protein homodimerization activity; copper ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen; protein heterodimerization activityBiological Process: melanin biosynthetic process; melanosome organization and biogenesis; melanocyte differentiation; acetoacetic acid metabolic processDisease: Albinism, Oculocutaneous, Type Iii; Skin/hair/eye Pigmentation, Variation In, 11 |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P17643 |
| NCBI GenInfo Identifier: | 12644141 |
| NCBI Gene ID: | 7306 |
| NCBI Accession: | P17643.2 |
| UniProt Secondary Accession: | P17643,P78468, P78469, Q13721, Q15679 |
| UniProt Related Accession: | P17643 |
| Molecular Weight: | 60,724 Da |
| NCBI Full Name: | 5,6-dihydroxyindole-2-carboxylic acid oxidase |
| NCBI Synonym Full Names: | tyrosinase-related protein 1 |
| NCBI Official Symbol: | TYRP1�� |
| NCBI Official Synonym Symbols: | TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN�� |
| NCBI Protein Information: | 5,6-dihydroxyindole-2-carboxylic acid oxidase; catalase B; DHICA oxidase; glycoprotein 75; melanoma antigen gp75 |
| UniProt Protein Name: | 5,6-dihydroxyindole-2-carboxylic acid oxidase |
| UniProt Synonym Protein Names: | Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1 |
| Protein Family: | 5,6-dihydroxyindole-2-carboxylic acid oxidase |
| UniProt Gene Name: | TYRP1�� |
| UniProt Entry Name: | TYRP1_HUMAN |
