The Human UB (Ubiquitin) CLIA Kit is a cutting-edge tool for precise measurement of ubiquitin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring dependable and reproducible results for various research purposes.Ubiquitin is a key protein that plays a vital role in protein degradation, cell signaling, and regulation of various cellular processes. Abnormal ubiquitin levels have been linked to conditions such as neurodegenerative disorders, cancer, and inflammatory diseases, making it a valuable biomarker for understanding these diseases and developing potential therapies.
With its advanced technology and reliable performance, the Human UB CLIA Kit is a valuable asset for researchers working in the fields of molecular biology, biochemistry, and biomedical sciences. Stay ahead in your research with this innovative kit from Assay Genie.
Product Name:
Human Ub (Ubiquitin) CLIA Kit
SKU:
HUES00713
Target:
Human Ub (Ubiquitin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Ub. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ub and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ub, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Ub. You can calculate the concentration of Human Ub in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
86-98
98-110
Average (%)
90
93
104
1:4
Range (%)
84-99
85-96
89-104
Average (%)
91
90
95
1:8
Range (%)
104-118
86-96
87-100
Average (%)
110
91
94
1:16
Range (%)
102-115
91-105
94-108
Average (%)
109
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.82
96.64
383.26
52.09
98.15
387.57
Standard deviation
5.19
9.0
33.88
5.92
8.2
43.56
C V (%)
10.21
9.31
8.84
11.36
8.35
11.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Ub concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ub in samples. No significant cross-reactivity or interference between Human Ub and analogues was observed.
