Human UBAP2 (Ubiquitin Associated Protein 2) CLIA Kit
The Human UBAP2 (Ubiquitin-Associated Protein 2) CLIA Kit is specifically designed for the accurate measurement of UBAP2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.UBAP2 is a protein that plays a vital role in the ubiquitination pathway, involved in regulating protein degradation and cellular processes.
Dysregulation of UBAP2 has been implicated in various diseases, including cancer and neurodegenerative disorders. As such, this kit is an invaluable tool for studying these conditions and potentially identifying new therapeutic targets.Overall, the Human UBAP2 CLIA Kit provides researchers with a powerful tool to accurately quantify UBAP2 levels and study its role in disease pathogenesis, offering valuable insights for developing novel treatment strategies.
Product Name:
Human UBAP2 (Ubiquitin Associated Protein 2) CLIA Kit
SKU:
HUES01233
Target:
Human UBAP2 (Ubiquitin Associated Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBAP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBAP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBAP2. You can calculate the concentration of Human UBAP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
98-113
98-111
Average (%)
105
104
106
1:4
Range (%)
98-112
90-102
96-109
Average (%)
103
97
102
1:8
Range (%)
90-103
85-100
90-101
Average (%)
96
91
95
1:16
Range (%)
101-118
92-105
97-112
Average (%)
109
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.96
638.23
1801.86
184.71
633.25
1830.49
Standard deviation
15.43
71.29
114.96
15.7
69.47
131.43
C V (%)
8.3
11.17
6.38
8.5
10.97
7.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UBAP2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UBAP2 in samples. No significant cross-reactivity or interference between Human UBAP2 and analogues was observed.
