The Human UBD (Ubiquitin D) CLIA Kit is a reliable and sensitive assay designed for the quantitative detection of UBD levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring precise and consistent results for various research applications.UBD is a key player in the ubiquitin-proteasome system, playing a crucial role in protein degradation, regulation of cellular processes, and immune response.
Dysregulation of UBD has been linked to various diseases including cancer, inflammatory disorders, and neurodegenerative diseases, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human UBD CLIA Kit provides researchers with a valuable tool to investigate the role of UBD in health and disease, offering a reliable and efficient method for measuring UBD levels in human samples.
Product Name:
Human UBD (Ubiquitin D) CLIA Kit
SKU:
HUES00714
Target:
Human UBD (Ubiquitin D)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBD. You can calculate the concentration of Human UBD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-120
86-99
96-112
Average (%)
109
91
102
1:4
Range (%)
95-111
95-109
86-96
Average (%)
102
103
91
1:8
Range (%)
90-103
97-110
90-101
Average (%)
96
104
96
1:16
Range (%)
96-113
85-99
89-100
Average (%)
103
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.69
7.74
21.26
2.88
8.38
21.46
Standard deviation
0.23
0.83
1.41
0.32
0.9
1.66
C V (%)
8.55
10.72
6.63
11.11
10.74
7.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UBD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UBD in samples. No significant cross-reactivity or interference between Human UBD and analogues was observed.
