Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit
The Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit is a powerful tool for the sensitive and specific detection of UBE3A levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high accuracy and reproducibility, making it ideal for a variety of research applications.UBE3A is a key protein involved in the ubiquitination process, playing a crucial role in protein degradation and regulation of various cellular processes. Dysregulation of UBE3A has been linked to neurodevelopmental disorders such as Angelman Syndrome, making it an important target for studying these conditions and exploring potential therapeutic interventions.
With its high sensitivity and reliability, the Human UBE3A ELISA Kit is a valuable tool for researchers studying ubiquitination pathways, neurodevelopmental disorders, and other related fields. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
