Human ucOC (Undercarboxylated Osteocalcin) ELISA Kit
The Human Undercarboxylated Osteocalcin (ucOC) ELISA Kit is specifically designed for the quantitative detection of undercarboxylated osteocalcin levels in various human biological samples. Undercarboxylated osteocalcin is an important biomarker associated with bone metabolism and may play a role in mineralization and the regulation of glucose and fat metabolism. Our ELISA kit from Assay Genie ensures exceptional sensitivity and specificity for accurate and reproducible results in your research.
With stringent quality control standards, the kit offers robust performance and user-friendly protocols, making it an excellent choice for studying undercarboxylated osteocalcin in physiological and pathological conditions. Trust Assay Genie's ELISA kit for precise quantification and comprehensive analysis of ucOC biomarkers in your studies.
Product Name:
Human ucOC (Undercarboxylated Osteocalcin) ELISA Kit
SKU:
AEES00148
Target:
Human ucOC (Undercarboxylated Osteocalcin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ucOC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ucOC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ucOC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ucOC. You can calculate the concentration of Human ucOC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
98-111
82-95
Average (%)
97
105
90
1:4
Range (%)
92-104
96-108
86-100
Average (%)
98
104
93
1:8
Range (%)
92-106
87-95
92-108
Average (%)
99
91
100
1:16
Range (%)
95-107
87-95
93-100
Average (%)
102
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
98
EDTA plasma (n=5)
91-101
98
Cell culture media (n=5)
93-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18
41.37
199.33
18.9
43.69
196.29
Standard deviation
0.96
2.04
9.45
0.95
2.04
8.17
C V (%)
5.33
4.93
4.74
5.03
4.67
4.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ucOC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ucOC in samples. No significant cross-reactivity or interference between Human ucOC and analogues was observed.
