The Human Urocortin-1 (UCN1) ELISA Kit from AssayGenie is a state-of-the-art assay designed for the precise and accurate measurement of Urocortin-1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an indispensable tool for researchers in various fields.Urocortin-1 is a peptide hormone that plays a crucial role in the regulation of stress responses, cardiovascular function, and metabolism.
Dysregulation of Urocortin-1 levels has been associated with various diseases including anxiety disorders, heart diseases, and metabolic disorders, highlighting its importance as a biomarker in research and clinical studies.Whether investigating the mechanisms underlying stress-related disorders or developing new therapeutic strategies for cardiovascular diseases, the Human Urocortin-1 (UCN1) ELISA Kit provides researchers with a powerful tool to advance their studies and make meaningful discoveries.
Product Name:
Human Urocortin 1 (UCN1) ELISA Kit
Product Code:
HUDL03040
Size:
96 Assays
Target:
UCN1
Synonyms:
UI, UROC, Urocortin
Detection Method:
Sandwich
Range:
15.6-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
