Human V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) ELISA Kit
The Human v-erb-a erythroblastic leukemia viral oncogene homolog 4 (ERBB4) ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of ERBB4 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for a variety of research applications.ERBB4 is a key protein that plays a vital role in cell growth, differentiation, and survival, making it a significant player in various diseases including cancer and neurodegenerative disorders.
By measuring ERBB4 levels, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially develop targeted therapies for them.Overall, the Human ERBB4 ELISA Kit offers a reliable and efficient solution for the quantitative analysis of ERBB4, enabling researchers to advance their understanding of disease pathogenesis and identify potential therapeutic targets.
Product Name:
Human V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
